Environmental Laboratory
Automating DNPH Derivatisation for Aldehyde Analysis
Apr 02 2012
Author: Dan Carrier on behalf of Element Materials Technology Ltd - Element Cambridge Life Sciences
Airborne aldehydes and ketones are collected by passing air through a cartridge containing 2,4-dinitrophenylhydrazine (DNPH). The Supelco cartridge is silica based and pre-coated with DNPH. Carbonyl compounds react with the DNPH to form derivatives in the form of hydrazones which are immobilised on the cartridge. Figure 1 shows the derivatistion of aldehydes and ketones using DNPH. These compounds can be easily eluted from the cartridge with acetonitrile and analysed by HPLC with UV detection.
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